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inactive erk  (Carna Inc)


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    Structured Review

    Carna Inc inactive erk
    Inactive Erk, supplied by Carna Inc, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/inactive+erk/Erk2/pmc06923413-321-19-21
    Average 94 stars, based on 26 article reviews
    inactive erk - by Bioz Stars, 2026-11
    94/100 stars

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    Activity Assay:

    Article Title: An ultrasensitive fiveplex activity assay for cellular kinases
    Article Snippet: Cysteine Sox Kinase Sensor kits were obtained for Akt (AssayQuant #AQT0536), IKKβ (AssayQuant #AQT0215), JNK1-3 (AssayQuant #AQT0365), and MK2 (AssayQuant #AQT0185). .. MEK activity was measured with a Cysteine Sox Kinase Sensor kit for ERK (AssayQuant #AQT0490) supplemented with 10 nM inactive ERK (Carna Biosciences #04-143-10). .. Peptide phosphorylation was monitored in black 384-well low-volume HiBase microplates (Greiner Bio-One #784076) in a 25 μL total volume containing 5 μL cell lysate (7–16 μg total protein) in 50 mM HEPES (pH 7.5), 0.01% Brij-35, 10 mM MgCl 2 , 1 mM DTT, 1 mM ATP, 6 μM PKA inhibitor peptide (Upstate #12-151), 6 μM PKC inhibitor peptide (Upstate #12–121), and 10 μM PhosphoSens Cysteine-Sox Kinase Sensor peptide substrate (AssayQuant).



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